A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity.
نویسندگان
چکیده
A novel time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) was developed. We used a straightforward strategy to modify a known ALK substrate into a peptide biosensor that can accommodate terbium luminescence sensitization upon its phosphorylation by ALK. Since this strategy is generalizable, this high-throughput screening compatible assay serves as an example for development of other kinase assays that employ terbium luminescence as a read-out.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 2 شماره
صفحات -
تاریخ انتشار 2015